培养蛋白用于狗粮配方的安全性和功能优势.pdf

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1、Anne Ballou1,Matt Longshaw2,Wendy Vandersteen31SIWA,Durham,North Carolina2Calysta(UK)Ltd,Cleveland3FirstMate,North Vancouver,British ColumbiaThe safety and microbiome impact of a novel single cell protein source in kennel and in-home trials.FeedKind PetCalysta founded in 2012 to re-establish and ref

2、ine technology developed in the 1990sAim to close protein gap in feed production through use of cultured proteinFeedKind Pet is grown at commercial scale in a closed loop fermenter,heat-killed,dried and bagged ready for use.Main bacteria in the consortia is MethylococcuscapsulatusAnimal StudiesModes

3、 of ActionInclusionPalatabilityFeedKind PetNaturalSustainableNon-GMOToxin-freeImprovedgut healthDigestive enzyme secretions,improved digestibilityImproved feedintake,health,and growthMicrobiomeImmuneactivationDiseaseResistanceStudy DesignComparisonpreencoded.pngIn-home StudyDog breeds:MixedDuration:

4、12 weeksDesign:Crossover study(6 test+6 control weeks)Animals:12(10M,2F)Doses:One(8%)Kennel StudyDog breeds:BeaglesDuration:8 monthsDesign:Safety study(6 test+2 control months)Animals:32(16M,16F)Doses:Three(4,6,&8%)Measures:Faecal score,BCS,intake,welfareStudy purpose:Research,consumer understanding

5、Measures:Faecal score,BCS,intake,welfare,clinical chemistry,urinalysis,hematology,digestibilityStudy purpose:Regulatory(GLP for GRASN submission)Comparison of Diet FormulationsDiets were not matched between trials,but proximates were similar:Protein 29%Fat 12-14%Fiber 4%Ash 8%Moisture 80%The rest of

6、 our focus today will be on the microbiomeWhat do these trials together tell us about FeedKinds impact on the GI microbiome?Feedkind is well-tolerated by dogs and their microbiomes Feedkind produces a consistently diverse and functional microbiome with traits consistent with microbiomes produced by