污染器械在再处理前的运输环境.pdf

1、Transportation Environment of Contaminated Instruments before ReprocessingKarin Bundgaard,Associated Professor,PhD,MScN,RNAalborg University Hospital&Aalborg UniversityPartners in crimePeter RubakGerhard KirmseSilke Haibt-WinandiThe contextUsagePre-treatmentSterilization and coolingInspection and wr

2、appingMechanical cleaning/disinfectionHandling and transportStorage before reuseIntroductionInternational infection control guidelines recommend-Reprocessing should commence immediately after use-Different recommendations for environment-Europe:mostly dry to minimize risk of corrosion-UK and America

3、:mostly moist to ensure efficient cleaning outcomes However-Recommendations are based on best practice and expert knowledge-Research in a real-life clinical setting is warrantedAimAim of the studyTo compare moist and dry transportation of surgical instruments in a real-life clinical setting-Protein

4、residues(cleanliness)-Corrosion(surface changes)MethodsData collection-Aarhus University Hospital-Basic instrument sets(54 and 39 surgical instruments)-Transportation-Dry:Abdominal Surgery Department-Moist:Orthopedic Spine Surgery Department Defining Moist transportation/storage-OR-Gauze(16 g,30 x45

5、 cm)soaked with 300 ml of sterile water-Closed containerMethodsProtein residue test-5-10 contaminated instruments from each tray-Washing process as is(stopped before the disinfection phase)-Elution process(included sonication)-High-sensitive BCA protein assay(Bicinchoninic Acid)Mechanical cleaning/d

6、isinfectionPre-cleaning2 minNeutral enzymatic cleaning(Getinge cleaner enzymatic 3 ml/l)5 minutes at 550 C(total cleaning time 8 minutes)1.RinseSoft water2.RinseSoft waterThermal disinfectionDemineralized water A0 3000Drying 15 minutesMethodsCalibration curve BCA Protein Assay KitMethodsSurface chan