利用 CRISPR 技术重振佛罗里达柑橘产业.pdf

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1、Yianni D.LagosYianni D.LagosPresident&CEOE:C:Bringing Back Florida Citrus using CRISPRBringing Back Florida Citrus using CRISPRSeptember 26,2025September 26,2025IntroductionIntroductionFlorida Citrus IndustryCRISPRGrower Tool:HLB-resistant CarriCeaT1 rootstockBringing Back Florida CitrusSource:USDA

2、National Agricultural Statistics Service 2018-2019,Dec.2024,Photos from UF/IFASFlorida Citrus Production 95%DeclineFlorida Citrus Production 95%DeclineHLBHLBMILLION CITRUS BOXESMILLION CITRUS BOXESFLORIDASFLORIDASDNADNACITRUS GREENINGS CITRUS GREENINGS FLORIDAFLORIDACommercialization of CRISPR Agric

3、ultural ProductsCommercialization of CRISPR Agricultural ProductsNatural Process and CRISPR Produce Same OutcomeNatural Process and CRISPR Produce Same OutcomeBeneficial Trait ChangesBeneficial Trait ChangesNaturally OccurringNaturally OccurringSpontaneous&Random CRISPRCRISPR-mediatedmediatedPredict

4、able&PreciseTarget Susceptibility SequenceTarget Susceptibility SequenceDouble-stranded BreakNon-Homologous End-JoiningDisease-resistance TraitNew Grower Tool:New Grower Tool:Multiple Modes of ActionNon-GMOFruitHLBResistance10,000X+CarrizoNormal GrowthCarriCea T1 Rootstock CarriCea T1 Rootstock Soil

5、ceas approach for diseaseSoilceas approach for disease-resistant citrus varietiesresistant citrus varietiesPathogens use effectors(weapons)to turn off plant host natural defense mechanisms(targets)to rapidly multiply in the plant.:EffectorsEffectors:Targets:TargetsPathogensPathogensPlantsPlantsSoilc

6、eas approach for diseaseSoilceas approach for disease-resistant citrus varietiesresistant citrus varietiesSoilcea disrupts effector-plant interactions using CRISPR precision breeding to restore the trees natural defense mechanisms to prevent HLB disease.:EffectorsEffectors:Targets:TargetsPathogensPa