基因驱动发展现状.pdf

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1、1State of Gene Drive DevelopmentJustin Overcash,Ph.D.2/27/25jovercashfnih.org2Overview of Talk A little bit of background Introduction to Gene Drives Ongoing Gene Drive projects Gene Drives to halt malaria Potential for Gene Drives in pest control Gene Drive release considerations Mission of the FNI

2、H and GeneConvene3Why am I giving a talk today?4What is a Gene Drive?5What do Gene Drives do?(Adapted from Alphey 2009)6What do Gene Drives do?(Adapted from Alphey 2009)7How do Gene Drives work:ReplicationCas9Repair via HDRMPMP8How are Gene Drives different than typical Genetically Engineered Organi

3、sms?Genetically Engineered CropsGene Drive Modified Organism Transient(in the ground and out)Immobile(Relatively)Confined Persistent Mobile Unconfined?9Gene Drives are not a MonolithPeople think Gene Drives areGene Drives are actually10What are other relevant Gene Drive characteristics?ThresholdRang

4、eAllele FrequencyGeneration TimeThresholdPersistenceAllele FrequencyGeneration TimeSuppressionReplacement11Each Gene Drive should be approached on a case-by-case basis12The case for Gene Drive mosquitoes94%of cases in Africa!13Gene Drive projects in AfricaSao Tome and PrincipeBurkina FasoUgandaTanza

5、nia14What kind of Gene Drives are being developed?Suppression Drive Targets sex determining/fertility genes Replicator Low Threshold Non-localized Range Limited Persistence(self-limited)Target Malaria Replacement Drive Introduces two Anti-malarial peptides Replicator Higher threshold(due to Non-Auto

6、nomous Drive)Localized(due to Non-Autonomous Drive)Extended PersistenceTransmission ZeroReplacement Drive Introduces two Anti-malarial peptidesReplicatorLow ThresholdNon-localized RangeExtended PersistenceUCMI15Potential Gene Drive targets16How close are we to the first field tra