巧克力斑病杀菌剂敏感性分析.pdf

1、Profiling fungicide sensitivity Profiling fungicide sensitivity of chocolate spotof chocolate spotSteven KildeaTeagasc Oak ParkNational Tillage Conference 2026Wednesday,28th January|Lyrath Hotel,Kilkennywww.teagasc.ie/tillagecon26 Chocolate Spot is a major disease of field beans Chocolate spot is ca

2、used by up-to four closely related Botrytis species Control currently reliant on 1-3 applications of fungicides from flowering onwards Limited number of fungicides registered for control Botrytis species are regarded as high risk of fungicide resistance Irish Chocolate Spot in 2023 exclusively cause

3、d by Botrytis fabaeB.fabaeIrish B.fabae Genetic analysis confirms all samples B.fabae Limited diversity amongst the isolates Only resistance to MBCs and iprodione reported in B.fabaeMutations known to cause fungicide resistance present in Irish Botrytis fabaeQoIs*SDHIsAzolesB.cinerea(Published data)

4、G143AB-P225H/L/FB-N230IB-H272Y/RC-P80H/LY136FN230KM231TG476SIrish B.fabae(n=46)G143AB-N230IB-H272RI232V(unknown impact)Frequency of mutations(%)59%86%100%*All sensitive isolates had an intron preventing G143AImpact of mutations on sensitivityUntreatedProline(azole)Solatenol(SDHI)Signum(SDHI&QoIComet

5、(QoI)Wild type(no mutations)SDHB H272RSDHB H272RCytB G143AMaximise preventative activity of fungicides Apply fungicides before disease symptoms(if possible)Ensure adequate canopy coverage Mix different modes of action Alternate between different MoA mixtures Fungicides must also provide rust controlAcknowledgmentsDr.Sheila AlvesJamie-Leigh CarterFiona HuttonWill discuss more in Workshop I