欧洲关于NGT育种的消息有多好?爱尔兰.pdf

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1、How good is the news from Europe on NGT breeding?Ewen MullinsTeagasc Oak ParkConventional v.NGT Breeding?2 Conventional breeding:Parent I x Parent II Evaluate 1000s of progeny Some with target trait(s)but many with undesirable traits Resource and time heavy to find high value variety NGT=New Genomic

2、 Techniques Ability to deliver an updated version of an existing variety with enhanced traits in a faster and more precise manner No crossing required with other varietyGenome Editing Several approaches to genome editing have been developed.CRISPR-Cas9=Clustered Regularly Interspaced Short Palindrom

3、ic Repeats and CRISPR-associated protein 9CisgenesisWild cultivarCommercial cultivarNGT derived cultivarGenome Editing CRISPR/Cas9(Targeted)Cisgenesis(Random/targeted)Cisgenesis:Accelerate the breeding process(transfer gene(s)from wild barley into a conventional barley variety)Gene Editing:Edit/disr

4、upt what is already there.No new DNA introduced into target varietyCommission proposal(2023)on plants obtained by certain new genomic techniques(NGTs)7What does it mean?Framework now in place for NGT derived plants Effective mid 2028 Distinguishes between two categories(NGT category I and II)NGT derived plants(and progeny)via Cat 1 equivalent to conventional varieties Positive development for crop breeding in Europe Can NGTs work?Desiree 11 x spraysDesiree 11 x spraysDesiree 11 x spraysDesiree 0 sprayDesiree 0 sprayNGT Desiree NGT DesireeNGT DesireeWorkshop IV for more details in afternoon

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