1、迈向内窥镜取样和培养的新型协调方法姓名:Lionel Pineau所属机构:EUROFINS1法国内窥镜微生物质量的演变020004000600080001000012000140001600018000200000.00%5.00%10.00%15.00%20.00%25.00%30.00%35.00%200420052006200720082009201020112012201320142015201620172018201920202021202220232024Number of samplesTotal(n=90311)Nombre dchantillonsEndoscopes au
2、 niveau dactionEndosocpes au niveau dalerte ou dactionPineau L.Endoscope reprocessing:Retrospective analysis of 90,311 samples.Endosc Int Open 2023;11:E247E257总计(n=120725)Endoscopes at the action levelEndoscopes at the action and alert level样本数量行动水平警报和行动水平2内窥镜取样和培养 实施内窥镜取样和培养程序对于定期评估内窥镜再处理是否足够至关重要。许
3、多权威机构和专业协会提出将内窥镜取样作为一项质量指标。文献研究表明,已经处理可以使用的内窥镜不合格率在0.4%至49.0%之间。(1)Instruction n DGOS/PF2/DGS/VSS1/2016/220 du 4 juillet 2016 relative au traitementdes endoscopes souples thermosensibles canaux au sein des lieux de soins.(2)Gastroenterological Society of Australia(GESA).Infection prevention and cont
4、rol in endoscopy 2021.(3)Advisory Board Cleaning and Disinfection Flexible Endoscopes(SFERD).Professional standard handbook.Flexible endoscopes cleaning and disinfection.2016.(4)FDA/CDC/ASM.Duodenoscope Surveillance Sampling and Culturing Protocols.2018.(5)Hygiene Requirements for the Reprocessing o
5、f Medical Devices.Bundesgesundheitsbl2012 55:12441310.法国,2016 年(1)美国,2023(4)澳大利亚,2021(2)德国,2018 年(5)01020304050602001200220032007200820092010201220152016201820202022不合规内窥镜的百分比%SaviucMineboisChiuGillespie RauwersBader Saliou OkamotoChomelMarkMoses Alfa Osborne 荷兰,2018(3)3内窥镜取样/培养方法的提取效率L.Pineau,M.Alf
6、a,C.Radix,Endoscope sampling and culturing methods,Journal of Hospital Infection,Volume 149,2024,Pages 36-45,ISSN 0195-6701,https:/doi.org/10.1016/j.jhin.2024.03.017.方法方法N5=0 CFUN2=8 CFUN3=2 CFUN4=2 CFU100 微升10 cfu/内窥镜微生物悬浮液采样溶液=1/=0N1=20 CFUObjective 目的目的I比较六种十二指肠镜取样和培养方法的有效性。.Principle 原则原则I比较五种取样